Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Jul;64(1):74-8.
doi: 10.1104/pp.64.1.74.

Investigations of vacuoles isolated from tobacco: I. Quantitation of nicotine

J A Saunders  1
Affiliations

Investigations of vacuoles isolated from tobacco: I. Quantitation of nicotine

J A Saunders. Plant Physiol. 1979 Jul.

Abstract

Nicotine was shown to be associated with mature vacuoles isolated from protoplasts of Nicotiana rustica. The vacuolar preparations also contained high levels of acid phosphatase, ATPase, and approximately 30% of the soluble protoplastic protein. The contamination of the vacuolar isolate by chlorophyll, succinate dehydrogenase, and NADPH cytochrome c reductase (markers for chloroplasts, mitochondria, and endoplasmic reticulum) was low. The enzymic activity associated with the vacuoles was not due to the exogenously supplied digestive enzymes used in the preparation of the protoplast. The relatively easy isolation of tobacco vacuoles makes this an excellent system for biochemical investigations of the vacuole.

PubMed Disclaimer

References

    1. Plant Physiol. 1977 Oct;60(4):629-34 - PubMed
    1. Anal Biochem. 1976 May 7;72:248-54 - PubMed
    1. Biochim Biophys Acta. 1977 Feb 14;465(1):110-7 - PubMed
    1. Plant Physiol. 1977 Jun;59(6):1098-103 - PubMed
    1. Plant Physiol. 1977 Jul;60(1):61-3 - PubMed

LinkOut - more resources