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. 1979 Aug;64(2):327-31.
doi: 10.1104/pp.64.2.327.

Phytochrome radioimmunoassay

R E Hunt  1 , L H Pratt
Affiliations

Phytochrome radioimmunoassay

R E Hunt et al. Plant Physiol. 1979 Aug.

Abstract

A phytochrome radioimmunoassay with a detection limit of about 2 nanograms has been developed. The radioimmunoassay does not suffer from the potential drawbacks of the commonly used spectral assay and requires less than 1 microliter of crude extract from dark-grown plants for quantitation of phytochrome. Measurement of phytochrome in crude extracts by radioimmunoassay gives values about 25% greater than those obtained by spectral assay. The amount of phytochrome detected in crude extracts of light-grown oats by radioimmunoassay is approximately 1% of that detected in comparable extracts from dark-grown oats. General interference by crude plant extracts with radioimmunoassays was also observed and corrected for.

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References

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