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. 1979 Aug;64(2):332-6.
doi: 10.1104/pp.64.2.332.

Phytochrome immunoaffinity purification

R E Hunt  1 , L H Pratt
Affiliations

Phytochrome immunoaffinity purification

R E Hunt et al. Plant Physiol. 1979 Aug.

Abstract

We have developed a phytochrome immunoaffinity purification procedure that yields undegraded oat (Avena sativa L., cv. Garry) phytochrome of greater than 98% purity within 2 hours when starting with a brushite-purified preparation. Immunoaffinity-purified phytochrome, except for its greater purity, is indistinguishable from conventionally purified phytochrome by gel exclusion chromatography, isoelectric focusing, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. We have also used the immunoaffinity technique to purify phytochrome from crude oat extracts, and from brushite-purified pea (Pisum sativum L., cv. Alaska) and rye (Secale cereale L., cv. Balbo) preparations.

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References

    1. Biochim Biophys Acta. 1972 Aug 28;276(2):454-74 - PubMed
    1. Plant Physiol. 1973 Jan;51(1):203-9 - PubMed
    1. Plant Physiol. 1979 Aug;64(2):327-31 - PubMed
    1. J Biol Chem. 1969 Aug 25;244(16):4406-12 - PubMed
    1. Biochim Biophys Acta. 1974 Sep 13;365(1):93-107 - PubMed

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