Photosynthetic oxygen reduction in isolated intact chloroplasts and cells in spinach

Abstract

The time course of light-induced O(2) exchange by isolated intact chloroplasts and cells from spinach was determined under various conditions using isotopically labeled O(2) and a mass spectrometer. In dark-adapted chloroplasts and cells supplemented with saturating amounts of bicarbonate, O(2) evolution began immediately upon illumination. However, this initial rate of O(2) evolution was counterbalanced by a simultaneous increase in the rate of O(2) uptake, so that little net O(2) was evolved or consumed during the first approximately 1 minute of illumination. After this induction (lag) phase, the rate of O(2) evolution increased 3- to 4-fold while the rate of O(2) uptake diminished to a very low level. Inhibition of the Calvin cycle, e.g. with dl-glyceraldehyde or iodoacetamide, had negligible effects on the initial rate of O(2) evolution or O(2) uptake; both rates were sutained for several minutes, and about balanced so that no net O(2) was produced. Uncouplers had an effect similar to that observed with Calvin cycle inhibitors, except that rates of O(2) evolution and photoreduction were stimulated 40 to 50%.These results suggest that higher plant phostosynthetic preparations which retain the ability to reduce CO(2) also have a significant capacity to photoreduce O(2). With near-saturating light and sufficient CO(2), O(2) reduction appears to take place primarily via a direct interaction between O(2) and reduced electron transport carriers, and occurs principally when CO(2)-fixation reactions are suboptimal, e.g. during induction or in the presence of Calvin cycle inhibitors. The inherent maximum endogenous rate of O(2) reduction is approximately 25 to 50% of the maximum rate of noncyclic electron transport coupled to CO(2) fixation. Although the photoreduction of O(2) is coupled to ion transport and/or phosphorylation, this process does not appear to supply significant amounts of ATP directly during steady-state CO(2) fixation in strong light.