Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Dec;64(6):1012-4.
doi: 10.1104/pp.64.6.1012.

Effects of pronase on isolated chloroplasts

L Fish  1 , V R Franceschi, C R Stocking
Affiliations

Effects of pronase on isolated chloroplasts

L Fish et al. Plant Physiol. 1979 Dec.

Abstract

Subjecting isolated spinach chloroplasts to mild proteolysis (10-minute incubation at 20 C in 500 micrograms per milliliter pronase) caused chloroplast clumping but did not affect their integrity as measured by their ability to carry out light stimulated, glycerate-3-P-dependent O(2) evolution. Transmission electron microscopy revealed no detectable differences between the control and treated plastids. Mild proteolysis inactivated exogenously added pyruvate kinase and should be a useful technique in certain enzyme distribution studies.

PubMed Disclaimer

References

    1. J Mol Biol. 1971 Jun 28;58(3):783-97 - PubMed
    1. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1095-101 - PubMed
    1. J Membr Biol. 1974;15(3):227-48 - PubMed
    1. Biochim Biophys Acta. 1976 Sep 21;448(1):103-13 - PubMed
    1. J Cell Biol. 1975 Dec;67(3):835-51 - PubMed

LinkOut - more resources