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. 1980 Jan;65(1):85-7.
doi: 10.1104/pp.65.1.85.

Assessment of cytoplasmic contaminations in isolated vacuole preparations

A Admon  1 , B Jacoby
Affiliations

Assessment of cytoplasmic contaminations in isolated vacuole preparations

A Admon et al. Plant Physiol. 1980 Jan.

Abstract

A method for testing the purity of isolated vacuoles is proposed. Preparations of isolated vacuoles often contain "impure vacuoles," namely, vacuoles contaminated with some cytoplasmic remnants. Such impure vacuoles are stained by the vital fluorescent dye fluorescein-diacetate. Vacuoles enclosed only by the tonoplast, "naked vacuoles," do not stain.

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References

    1. Plant Physiol. 1976 Nov;58(5):656-62 - PubMed
    1. Proc Natl Acad Sci U S A. 1966 Jan;55(1):134-41 - PubMed

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