Effect of NAD on Malate Oxidation in Intact Plant Mitochondria
- PMID: 16661409
- PMCID: PMC440570
- DOI: 10.1104/pp.66.2.225
Effect of NAD on Malate Oxidation in Intact Plant Mitochondria
Abstract
Potato tuber mitochondria oxidizing malate respond to NAD(+) addition with increased oxidation rates, whereas mung bean hypocotyl mitochondria do not. This is traced to a low endogenous content of NAD(+) in potato mitochondria, which prove to take up added NAD(+). This mechanism concentrates NAD(+) in the matrix space. Analyses for oxaloacetate and pyruvate (with pyruvate dehydrogenase blocked) are consistent with regulation of malate oxidation by the internal NAD(+)/NADH ratio.
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