Effect of irradiance on development of apparent nitrogen fixation and photosynthesis in soybean

Abstract

Soybeans grown with 2 millimolar NO(3) (-), which optimized apparent N(2) fixation by Rhizobium symbionts, showed significantly different rates of apparent photosynthesis and C(2)H(2) reduction during seedling development at two irradiances. Those physiological processes were lower for several weeks in plants grown at 1,500 microeinsteins per meter(2) per second than in those exposed to 700 microeinsteins per meter(2) per second. The irradiance-induced retardation was evident in short-term rates of apparent photosynthesis and N(2) fixation, as well as in measures of dry matter and total N accumulation. In spite of their previously inhibited development, plants grown at 1,500 microeinsteins per meter(2) per second were indistinguishable by day 28 from those exposed to 700 microeinsteins per meter(2) per second in terms of whole-shoot CO(2)-exchange rate; by day 35 they were identical in terms of whole-plant C(2)H(2)-reduction rate. On day 38 there was no significant difference in dry weight or N content between treatments. Shifting plants between irradiance treatments on day 21 showed that the higher irradiance also had a short-term inhibitory effect on C(2)H(2) reduction. The fact that 16 millimolar NO(3) (-) prevented the continuous exposure to 1,500 microeinsteins per meter(2) per second from inhibiting apparent photosynthesis suggested that seedlings grown on 2 millimolar NO(3) (-) with Rhizobium were N-limited. Although rates of apparent photosynthesis were similar by day 28, the additional week required to produce equal rates of apparent N(2) fixation between irradiance treatments showed that physiological adaptations of shoots, as well as photosynthesis per se, can affect root nodule activity.