Movement of C-compounds from Maternal Tissue into Maize Seeds Grown in Vitro

Abstract

Uptake from nutrient media into the cob and translocation of various (14)C-compounds from maternal tissue (cob) into developing maize seeds was examined by using caryopsis cultures. Based on relative (14)C concentrations in the cob and the endosperm, it was concluded that the relative efficiencies of movement of amino acids (leucine, phenylalanine, proline), vitamins (thiamine HCl, nicotinic acid), and nucleic acid bases (adenine, thymine) from the cob to the endosperm were 11 to 250 times lower than that of sucrose. Thiamine was unique in that it was concentrated in the embryo at a level that was almost 10 times higher than in the endosperm. The absence of auxotrophic mutants requiring an organic supplement in higher plants (other than thiamine auxotrophs) may be explained by inadequate translocation of these essential metabolites into the mutant zygotes (embryos) to enable their development to mature seeds.