doi: 10.1104/pp.67.3.467.
Role of Glutamate-oxaloacetate Transaminase and Malate Dehydrogenase in the Regeneration of NAD for Glycine Oxidation by Spinach leaf Mitochondria
Affiliations
- PMID: 16661695
- PMCID: PMC425706
- DOI: 10.1104/pp.67.3.467
Item in Clipboard
Role of Glutamate-oxaloacetate Transaminase and Malate Dehydrogenase in the Regeneration of NAD for Glycine Oxidation by Spinach leaf Mitochondria
Plant Physiol.
1981 Mar.
Abstract
During glycine oxidation by spinach leaf mitochondria, oxygen consumption showed a strong and transient inhibition upon addition of oxaloacetate or aspartate plus alpha-ketoglutarate. During the course of the inhibition, aspartate and alpha-ketoglutarate were stoichiometrically transformed into malate and glutamate.It is concluded that oxaloacetate formed by transamination is reduced by the malate dehydrogenase, which allows the regeneration of NAD(+) for glycine oxidation and, thus, by-passes the respiratory chain. Efficiency of a malate-glutamate/aspartate-alpha-ketoglutarate shuttle upon illumination and under in vivo conditions is discussed.
Similar articles
-
CONTROL OF GLUTAMATE OXIDATION IN BRAIN AND LIVER MITOCHONDRIAL SYSTEMS.Biochem J. 1965 May;95(2):497-508. doi: 10.1042/bj0950497. Biochem J. 1965. PMID: 14340100 Free PMC article.
-
Effect of bicarbonate and oxaloacetate on malate oxidation by spinach leaf mitochondria.Biochim Biophys Acta. 1980 Feb 8;589(2):176-89. doi: 10.1016/0005-2728(80)90036-5. Biochim Biophys Acta. 1980. PMID: 7356982
-
Suppression of the mitochondrial oxidation of (-)-palmitylcarnitine by the malate-aspartate and alpha-glycerophosphate shuttles.J Biol Chem. 1976 Jan 25;251(2):277-84. J Biol Chem. 1976. PMID: 1245472
-
Acute sources of mitochondrial NAD+ during respiratory chain dysfunction.Exp Neurol. 2020 May;327:113218. doi: 10.1016/j.expneurol.2020.113218. Epub 2020 Feb 5. Exp Neurol. 2020. PMID: 32035071 Review.
-
Glutamine-Glutamate Cycle Flux Is Similar in Cultured Astrocytes and Brain and Both Glutamate Production and Oxidation Are Mainly Catalyzed by Aspartate Aminotransferase.Biology (Basel). 2017 Feb 24;6(1):17. doi: 10.3390/biology6010017. Biology (Basel). 2017. PMID: 28245547 Free PMC article. Review.
Cited by
-
Characterization of dicarboxylate stimulation of ammonia, glutamine, and 2-oxoglutarate-dependent o(2) evolution in isolated pea chloroplasts.Plant Physiol. 1983 Jun;72(2):291-6. doi: 10.1104/pp.72.2.291. Plant Physiol. 1983. PMID: 16662995 Free PMC article.
-
Purification of peroxisomes and mitochondria from spinach leaf by percoll gradient centrifugation.Plant Physiol. 1984 Jul;75(3):670-4. doi: 10.1104/pp.75.3.670. Plant Physiol. 1984. PMID: 16663685 Free PMC article.
-
The photorespiratory hydrogen shuttle. Synthesis of phthalonic acid and its use in the characterization of the malate/aspartate shuttle in pea (Pisum sativum) leaf mitochondria.Biochem J. 1987 Aug 1;245(3):669-75. doi: 10.1042/bj2450669. Biochem J. 1987. PMID: 3663185 Free PMC article.
-
Uncoupling proteins 1 and 2 (UCP1 and UCP2) from Arabidopsis thaliana are mitochondrial transporters of aspartate, glutamate, and dicarboxylates.J Biol Chem. 2018 Mar 16;293(11):4213-4227. doi: 10.1074/jbc.RA117.000771. Epub 2018 Jan 25. J Biol Chem. 2018. PMID: 29371401 Free PMC article.
-
Cold acclimation alters DNA methylation patterns and confers tolerance to heat and increases growth rate in Brassica rapa.J Exp Bot. 2017 Feb 1;68(5):1213-1224. doi: 10.1093/jxb/erw496. J Exp Bot. 2017. PMID: 28158841 Free PMC article.
References
LinkOut - more resources
Full Text Sources
