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. 1982 Jan;69(1):37-40.
doi: 10.1104/pp.69.1.37.

Characteristics of teratomas regenerated in vitro from octopine-type crown gall

L D Owens  1
Affiliations

Characteristics of teratomas regenerated in vitro from octopine-type crown gall

L D Owens. Plant Physiol. 1982 Jan.

Abstract

Crown galls induced by infection of tobacco plants with Agrobacterium tumefaciens strain C58-Cl(pTiB6S3) were excised and cultured in vitro. After about one year of culture on medium-lacking phytohormones, two noncloned lines spontaneously formed shoots. Leaf explants from shoots of tumor-line T5 were capable of growing on hormone-free medium, and the resulting mixture of organized and unorganized tissue synthesized octopine. Detached leaves from T5 shoots also synthesized octopine. These results establish that shoots from this octopine-type tumor contain transformed cells and are true crown-gall teratomas.

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