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. 1982 Aug;70(2):410-3.
doi: 10.1104/pp.70.2.410.

Identification of the leaf vacuole as a major nitrate storage pool

R C Granstedt  1 , R C Huffaker
Affiliations

Identification of the leaf vacuole as a major nitrate storage pool

R C Granstedt et al. Plant Physiol. 1982 Aug.

Abstract

Highly purified vacuoles were isolated from protoplasts derived from green barley (Hordeum vulgare var. Numar) leaves, in order to determine their role as a NO(3) (-) storage sink. alpha-Mannosidase and acid phosphatase activities were used as markers to identify vacuoles, alpha-mannosidase being the more suitable. Nitrate and alpha-mannosidase, which were released from vacuoles destroyed during lysis of protoplasts, moved at unequal rates in the density gradient used for vacuole isolation. Purified vacuoles retained less NO(3) (-) than alpha-mannosidase during a single washing. Empirically determined corrections were used to account for NO(3) (-) movement in estimating the percentage of total cellular nitrate found in the vacuole. Vacuoles from plants grown in the presence of NO(3) (-) contained 58% of the total cellular NO(3) (-) and therefore represent a major NO(3) (-) storage pool.

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References

    1. Plant Physiol. 1979 Jun;63(6):1205-9 - PubMed
    1. Plant Physiol. 1979 Dec;64(6):1114-20 - PubMed
    1. Plant Physiol. 1978 Feb;61(2):154-7 - PubMed
    1. Plant Physiol. 1980 May;65(5):871-9 - PubMed
    1. Plant Physiol. 1982 Mar;69(3):575-9 - PubMed

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