Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1982 Oct;70(4):1156-61.
doi: 10.1104/pp.70.4.1156.

A P Nuclear Magnetic Resonance Study of Intracellular pH of Plant Cells Cultivated in Liquid Medium

J B Martin  1 , R Bligny, F Rebeille, R Douce, J J Leguay, Y Mathieu, J Guern
Affiliations

A P Nuclear Magnetic Resonance Study of Intracellular pH of Plant Cells Cultivated in Liquid Medium

J B Martin et al. Plant Physiol. 1982 Oct.

Abstract

(31)P nuclear magnetic resonance has been used to study the vacuolar and cytoplasmic pH of Acer pseudoplatanus, Catharanthus roseus, and Glycine max cells grown as cell suspensions. The adaptation of this technique to plant cells grown in liquid medium is described with emphasis on the removal of Mn(2+) and phosphate from the extracellular medium and on providing the O(2) supply of the cells in the nuclear magnetic resonance tube and the various problems of calibration. Aerobic and anaerobic cells show large differences in their glucose-6-phosphate, their cytoplasmic inorganic phosphate pools, and their cytoplasmic pH. Differences in the relative sizes of the cytoplasmic and vacuolar inorganic phosphate pools have been observed for the three cell strains studied.

PubMed Disclaimer

References

    1. J Biol Chem. 1979 Feb 25;254(4):1170-7 - PubMed
    1. Plant Physiol. 1971 Jan;47(1):48-52 - PubMed
    1. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2125-9 - PubMed
    1. Plant Physiol. 1968 Jun;43(6):948-52 - PubMed
    1. Biochemistry. 1979 Oct 16;18(21):4487-99 - PubMed

LinkOut - more resources