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. 1982 Oct;70(4):1213-7.
doi: 10.1104/pp.70.4.1213.

Isolation and purification of intact peroxisomes from green leaf tissue

M R Schmitt  1 , G E Edwards
Affiliations

Isolation and purification of intact peroxisomes from green leaf tissue

M R Schmitt et al. Plant Physiol. 1982 Oct.

Abstract

Intact peroxisomes were prepared from green leaves of a number of C(3) species, both monocots and dicots. A protoplast extract from which chloroplasts have been removed by a 1-minute 10,000g centrifugation was applied to a step gradient of 5, 15, 30, and 45% Percoll containing 0.5 molar sucrose, 0.1% BSA, and 25 millimolar Hepes-KOH (pH 7.5). After centrifugation, a peroxisomal fraction with low contamination by chloroplastic and mitochondrial markers was recovered from the 30/45% Percoll interface. This fraction was passed through a Sepharose 2B column to remove the Percoll which resulted in a peroxisomal preparation exhibiting high intactness (estimated from enzyme latency) and stability.

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References

    1. Plant Physiol. 1982 Apr;69(4):916-20 - PubMed
    1. J Biol Chem. 1970 Aug 10;245(15):3831-9 - PubMed
    1. Plant Physiol. 1969 Feb;44(2):295-300 - PubMed
    1. Anal Biochem. 1978 Mar;85(1):271-5 - PubMed
    1. Plant Physiol. 1981 Sep;68(3):755-61 - PubMed

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