Limiting Factors in Photosynthesis: IV. Iron Stress-Mediated Changes in Light-Harvesting and Electron Transport Capacity and its Effects on Photosynthesis in Vivo

Abstract

Using iron stress to reduce the total amount of light-harvesting and electron transport components per unit leaf area, the influence of light-harvesting and electron transport capacity on photosynthesis in sugar beet (Beta vulgaris L. cv F58-554H1) leaves was explored by monitoring net CO(2) exchange rate (P) in relation to changes in the content of Chl.In most light/CO(2) environments, and especially those with high light (>/=1000 microeinsteins photosynthetically active radiation per square meter per second) and high CO(2) (>/=300 microliters CO(2) per liter air), P per area was positively correlated with changes in Chl (a + b) content (used here as an index of the total amount of light-harvesting and electron transport components). This positive correlation of P per area with Chl per area was obtained not only with Fe-deficient plants, but also over the normal range of variation in Chl contents found in healthy, Fe-sufficient plants. For example, light-saturated P per area at an ambient CO(2) concentration close to normal atmospheric levels (300 microliters CO(2) per liter air) increased by 36% with increase in Chl over the normal range, i.e. from 40 to 65 micrograms Chl per square centimeter. Iron deficiency-mediated changes in Chl content did not affect dark respiration rate or the CO(2) compensation point. The results suggest that P per area of sugar beet may be colimited by light-harvesting and electron transport capacity (per leaf area) even when CO(2) is limiting photosynthesis as occurs under field conditions.