Heterogeneity of pumpkin ribosomal DNA
- PMID: 16662953
- PMCID: PMC1066188
- DOI: 10.1104/pp.72.1.166
Heterogeneity of pumpkin ribosomal DNA
Abstract
The ribosomal DNA (rDNA) of Cucurbita pepo L. has been found to consist of tandemly arrayed repeat units, most of which are 10 kilobases in length. Thirty-six repeat units, cloned into the HindIII site of pACYC 177, fall into seven classes which differ from each other in length and/or nucleotide sequence. Most of the heterogeneity occurs in noncoding portions of the repeat unit although there is some nucleotide sequence variation in the coding portion as well. Heterogeneity of base modification was observed in genomic rDNA of which two examples are: (a) all of the repeat units have three BamHI sites, one of which is unavailable for restriction in about half of the units and (b) all of the CCGG sites except one are methylated at the internal cytidine in many of the units; a second site is unmethylated in some of the units and in a very few units a third site remains unmethylated.
Similar articles
-
Heterogeneity and organization of the ribosomal RNA genes of Cucurbita maxima.Plant Mol Biol. 1990 Jun;14(6):927-33. doi: 10.1007/BF00019390. Plant Mol Biol. 1990. PMID: 2102876
-
Novel variants of the 5S rRNA genes in Eruca sativa.Genome. 1994 Feb;37(1):121-8. doi: 10.1139/g94-015. Genome. 1994. PMID: 8181733
-
Different AT-rich satellite DNAs in Cucurbita pepo and Cucurbita maxima.Theor Appl Genet. 1986 Nov;73(1):129-35. doi: 10.1007/BF00273729. Theor Appl Genet. 1986. PMID: 24240758
-
Nucleotide sequence of the BamHI repetitive sequence, including the HindIII fundamental unit, as a possible mobile element from the Japanese monkey Macaca fuscata.J Mol Evol. 1986;23(3):200-4. doi: 10.1007/BF02115576. J Mol Evol. 1986. PMID: 3027353
-
The Cucurbita maxima ribosomal DNA intergenic spacer has a complex structure.Gene. 1989 Aug 15;80(2):239-48. doi: 10.1016/0378-1119(89)90288-6. Gene. 1989. PMID: 2583512
Cited by
-
Structure of melon rDNA and nucleotide sequence of the 17-25S spacer region.Theor Appl Genet. 1988 Nov;76(5):673-80. doi: 10.1007/BF00303512. Theor Appl Genet. 1988. PMID: 24232344
-
Ribosomal RNA genes of Phaseolus coccineus. I.Plant Mol Biol. 1992 Apr;18(6):1073-82. doi: 10.1007/BF00047710. Plant Mol Biol. 1992. PMID: 1600143
-
Ribosomal RNA genes in plants: variability in copy number and in the intergenic spacer.Plant Mol Biol. 1987 Sep;9(5):509-20. doi: 10.1007/BF00015882. Plant Mol Biol. 1987. PMID: 24277137
-
The structure of the maize ribosomal DNA spacer region.Nucleic Acids Res. 1986 Jun 25;14(12):4953-68. doi: 10.1093/nar/14.12.4953. Nucleic Acids Res. 1986. PMID: 3725589 Free PMC article.
-
Structure and variation in ribosomal RNA genes of pea : Characterization of a cloned rDNA repeat and chromosomal rDNA variants.Plant Mol Biol. 1987 Jan;8(1):3-12. doi: 10.1007/BF00016429. Plant Mol Biol. 1987. PMID: 24302519
References
LinkOut - more resources
Full Text Sources