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. 1983 Jul;72(3):809-12.
doi: 10.1104/pp.72.3.809.

An endogenous alpha-amylase inhibitor in barley kernels

R J Weselake  1 , A W Macgregor, R D Hill
Affiliations

An endogenous alpha-amylase inhibitor in barley kernels

R J Weselake et al. Plant Physiol. 1983 Jul.

Abstract

Barley (Hordeum distichum cv Klages) kernels were shown to contain a factor that converted malted barley alpha-amylase II to the alpha-amylase III form. After purification by ammonium sulfate fractionation, ion exchange chromatography on DEAE-Sephacel, and gel-filtration on Bio Gel P60, the factor gave a single band of protein on isoelectric focusing. The purified factor inhibited hydrolysis of soluble starch by alpha-amylase II from malted barley and germinated wheat (Triticum aestivum cv Neepawa). However, alpha-amylase I from these cereals was not affected. The inhibitor was not dialyzable and was retained by a PM 10 ultrafiltration membrane suggesting a molecular weight greater than 10,000 daltons. Heat treatment of the inhibitor at 70 degrees C for 15 minutes at pH 5.5 and 8.0 resulted in considerable loss of inhibitory activity.

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