Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1983 Oct;73(2):398-406.
doi: 10.1104/pp.73.2.398.

Purification and separation of plant gibberellins from their precursors and glucosyl conjugates

M Koshioka  1 , K Takeno, F D Beall, R P Pharis
Affiliations

Purification and separation of plant gibberellins from their precursors and glucosyl conjugates

M Koshioka et al. Plant Physiol. 1983 Oct.

Abstract

A procedure using two small preparative columns (in sequence) of C(18) reverse phase Bondapak B material with methanolic extracts of plant tissue (Pisum sativum L., Malus domestica Borkh., Pimpinella anisum L.) yields two fractions: (i) gibberellin (GA) precursors, and (ii) free GA/GA methyl esters (GA-Me)/GA glucosyl conjugates. The discrete separation of (iii) free GA/GA-Me from (iv) GA glucosyl conjugates is then accomplished by a combination of differential solvent solubility and SiO(2) partition chromatography. All fractions are almost pigment free, and appreciable dry weight purification was accomplished for the GA precursor and free GA/GA-Me fractions. Solvent volumes can be kept low, no buffer salts are introduced, and each fraction (i, iii, iv) can be subjected directly to preparative or analytical reverse phase C(18) high performance liquid chromatography without recourse to solvent partitioning, and often without further purification.

PubMed Disclaimer

References

    1. Plant Physiol. 1980 Feb;65(2):218-21 - PubMed
    1. Science. 1971 May 21;172(3985):856-7 - PubMed
    1. Plant Physiol. 1980 Apr;65(4):623-6 - PubMed
    1. Experientia. 1964 Feb 15;20(2):89-90 - PubMed
    1. Plant Physiol. 1971 Jun;47(6):821-7 - PubMed

LinkOut - more resources