Mechanism of Short Term Fe Reduction by Roots : Evidence against the Role of Secreted Reductants

Abstract

The hypothesized role of secreted reducing compounds in Fe(III) reduction has been examined with Fe-deficient peanuts (Arachis hypogaea L. cv A124B). Experiments involved the exposure of roots to (a) different gas mixtures, (b) carbonyl cyanide m-chlorophenylhydrazone (CCCP), and (c) agents which impair membrane integrity.Removing roots from solution and exposing them to air or N(2) for 10 minutes did not result in any accumulation in the free space of compounds capable of increasing rates of Fe(III) reduction when roots were returned to solutions. On the contrary, exposing roots to N(2) decreased rates of Fe(III) reduction. CCCP also decreased rates of Fe(III) reduction.Acetic acid and ethylenediaminetetraacetic acid (disodium salt) (EDTA) impaired the integrity and function of the plasma membranes of roots of Fe-deficient peanuts. That is, in the presence of acetic acid or EDTA, there was an efflux of K(+) from the roots; K(+) ((86)Rb) uptake was also impaired. Acetic acid increased the efflux from the roots of compounds capable of reducing Fe(III). However, both acetic acid and EDTA caused rapid decreases in rates of Fe(III) reduction by the roots. In addition to peanuts, acetic acid also decreased rates of Fe(III) reduction by roots of Fe-deficient sunflowers (Helianthus annuus L. cv Sobrid) but not maize (Zea mays L. cv Garbo).These results suggest that, at least in the short term, the enhanced Fe(III) reduction by roots of Fe-deficient plants is not due to the secretion of reducing compounds.