Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1984 Jul;75(3):865-8.
doi: 10.1104/pp.75.3.865.

Analysis of the Complexity and Diversity of mRNAs from Pollen and Shoots of Tradescantia

R P Willing  1 , J P Mascarenhas
Affiliations

Analysis of the Complexity and Diversity of mRNAs from Pollen and Shoots of Tradescantia

R P Willing et al. Plant Physiol. 1984 Jul.

Abstract

The mRNAs of the mature pollen grain of Tradescantia paludosa at anesthesia and of vegetative shoots have been compared by analyzing the kinetics of hybridization between homologous and heterologous reactions of cDNA to poly(A)RNA in excess. The mRNAs in pollen can be divided into three abundance classes with complexities of 5.2 x 10(4), 1.6 x 10(6), and 2.1 x 10(7) nucleotides. The three classes are made up of sequences that constitute 15, 60, and 24% of the mRNAs and each sequence is present on an average at 26,000, 3,400, and 100 copies, respectively, per pollen grain. About 20,000 different genes are expressed in pollen as compared to about 30,000 in vegetative shoots. Estimates have been made of pollen mRNA sequences shared with those of shoot tissue and of shoot sequences common to those in pollen.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1977 Jun;4(6):1727-37 - PubMed
    1. Nature. 1974 Jul 19;250(463):199-204 - PubMed
    1. Methods Enzymol. 1980;65(1):65-74 - PubMed
    1. Cell. 1978 May;14(1):123-31 - PubMed
    1. Prog Nucleic Acid Res Mol Biol. 1976;19:253-60 - PubMed

LinkOut - more resources