Potassium Transport in Corn Roots : III. Perturbation by Exogenous NADH and Ferricyanide

Abstract

It has recently been reported that plasmalemma electron transport may be involved in the generation of H(+) gradients and the uptake of ions into root tissue. We report here on the influence of extracellular NADH and ferricyanide on K(+) ((86)Rb(+)) influx, K(+) ((86)Rb(+)) efflux, net apparent H(+) efflux, and O(2) consumption in 2-centimeter corn (Zea mays [A632 x Oh43]) root segments and intact corn roots. In freshly excised root segments, NADH had no effect on O(2) consumption and K(+) uptake. However, after the root segments were given a 4-hour wash in aerated salt solution, NADH elicited a moderate stimulation in O(2) consumption but caused a dramatic inhibition of K(+) influx. Moreover, net apparent H(+) efflux was significantly inhibited following NADH exposure in 4-hour washed root segments.Exogenous ferricyanide inhibited K(+) influx in a similar fashion to that caused by NADH, but caused a moderate stimulation of net H(+) efflux. Additionally, both reagents substantially altered K(+) efflux at both the plasmalemma and tonoplast.These complex results do not lend themselves to straightforward interpretation and are in contradiction with previously published results. They suggest that the interaction between cell surface redox reactions and membrane transport are more complex than previously considered. Indeed, more than one electron transport system may operate in the plasmalemma to influence, or regulate, a number of transport functions and other cellular processes. The results presented here suggest that plasmalemma redox reactions may be involved in the regulation of ion uptake and the ;wound response' exhibited by corn roots.