Cell surfaces in plant-microorganism interactions : v. Elicitors of fungal and of plant origin trigger the synthesis of ethylene and of cell wall hydroxyproline-rich glycoprotein in plants

Abstract

Treatment of melon hypocotyls or petioles with an elicitor from Colletotrichum lagenarium, a fungal pathogen of melons, causes an initial transitory inhibition of protein synthesis and, after 18 hours, induces the synthesis of a plant cell wall hydroxyproline-rich glycoprotein (HRGP). Microgram amounts of elicitor are sufficient for maximum elicitation of HRGP when the elicitor is injected into hypocotyls. High elicitor concentrations have a strong inhibitory effect on total protein synthesis. Ethylene is increased early in elicitor-treated plant material, and may be involved in HRGP elicitation. In the presence of aminoethoxyvinylglycine, an inhibitor of ethylene synthesis, both elicitor-induced ethylene and elicitor-induced HRGP are inhibited. On the other hand, 1-aminocyclopropane-1-carboxylic acid, the direct precursor of ethylene, triggers the synthesis of HRGP to the same extent as the elicitor of C. lagenarium, and partly restores in elicitor-treated petioles the synthesis of ethylene and of HRGP after previous inhibition by aminoethoxyvinylglycine. Elicitation of HRGP occurs in other systems, such as soybeans when inoculated with an elicitor from Phytophtora megasperma f. sp. glycinea, and when melons are incubated with an elicitor isolated from their cell walls.