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. 1985 May;78(1):178-82.
doi: 10.1104/pp.78.1.178.

Analysis of Leaf Proteins by Two-Dimensional Gel Electrophoresis: Protease Action as Exemplified by Ribulose Bisphosphate Carboxylase/ Oxygenase Degradation and Procedure to Avoid Proteolysis during Extraction

C C des Francs  1 , H Thiellement, D de Vienne
Affiliations

Analysis of Leaf Proteins by Two-Dimensional Gel Electrophoresis: Protease Action as Exemplified by Ribulose Bisphosphate Carboxylase/ Oxygenase Degradation and Procedure to Avoid Proteolysis during Extraction

C C des Francs et al. Plant Physiol. 1985 May.

Abstract

A previous genetic analysis by two-dimensional electrophoresis of wheat leaf proteins led us to hypothesize that several polypeptides were degradation products of the large subunit of ribulose bisphosphate carboxylase/oxygenase (EC 4.1.1.39).VARIOUS EXTRACTION PROCEDURES INCLUDING THE USE OF PROTEINASE INHIBITORS ALLOWED US TO: (a) confirm that the suspected polypeptides are indeed degradation products; (b) find out under which conditions the proteinases act; (c) find a method which prevents degradation, by boiling the extract in the presence of sodium dodecyl sulfate and 2-mercaptoethanol.

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References

    1. Plant Physiol. 1979 Nov;64(5):884-7 - PubMed
    1. J Biol Chem. 1975 May 25;250(10):4007-21 - PubMed
    1. Plant Physiol. 1981 Oct;68(4):930-6 - PubMed
    1. Plant Physiol. 1978 Apr;61(4):501-5 - PubMed
    1. Plant Physiol. 1978 Oct;62(4):604-8 - PubMed