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. 1985 May;78(1):89-91.
doi: 10.1104/pp.78.1.89.

Separation and quantitation of polyamines in plant tissue by high performance liquid chromatography of their dansyl derivatives

M A Smith  1 , P J Davies
Affiliations

Separation and quantitation of polyamines in plant tissue by high performance liquid chromatography of their dansyl derivatives

M A Smith et al. Plant Physiol. 1985 May.

Abstract

High performance liquid chromatography in combination with fluorescence spectrophotometry can be used to separate and quantitate polyamines (putrescine, cadaverine, spermidine, spermine), prepared as their dansyl derivatives, from plant tissue. The procedure gives sensitive and consistent results for polyamine determinations in plant tissue. In a standard mixture, the minimal detection level was less than 1 picomole of polyamines.

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