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. 1985 Jul;78(3):652-4.
doi: 10.1104/pp.78.3.652.

Pollen sporoplasts: dissolution of pollen walls

F A Loewus  1 , B G Baldi, V R Franceschi, L D Meinert, J J McCollum
Affiliations

Pollen sporoplasts: dissolution of pollen walls

F A Loewus et al. Plant Physiol. 1985 Jul.

Abstract

4-Methylmorpholine N-oxide monohydrate (MMNO.H(2)O), a potent solvent for polysaccharides, is an effective vehicle for release of membrane-enclosed male gametophytes (sporoplasts) from spore walls. This release occurs in minutes when pollen (Lilium longiflorum Thunb.) is suspended in a melt of MMNO.H(2)O at 75 degrees C. Continued heating at 75 degrees C leads to distintegration of the exine ;shell' which coalesces into immiscible globules in the MMNO melt. These observations provide a general procedure for preparation of pollen sporoplasts and sporoplast outer membranes, and offer a new method for dissolving the sporopollenin component of the spore wall.

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References

    1. Plant Physiol. 1978 Aug;62(2):280-3 - PubMed
    1. Stain Technol. 1972 May;47(3):137-9 - PubMed

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