Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1986 Jan;80(1):202-5.
doi: 10.1104/pp.80.1.202.

Characterization of 12-oxo-phytodienoic Acid reductase in corn: the jasmonic Acid pathway

B A Vick  1 , D C Zimmerman
Affiliations

Characterization of 12-oxo-phytodienoic Acid reductase in corn: the jasmonic Acid pathway

B A Vick et al. Plant Physiol. 1986 Jan.

Abstract

12-Oxo-phytodienoic acid reductase, an enzyme of the biosynthetic pathway that converts linolenic acid to jasmonic acid, has been characterized from the kernel and seedlings of corn (Zea mays L.). The molecular weight of the enzyme, estimated by gel filtration, was 54,000. Optimum enzyme activity was observed over a broad pH range, from pH 6.8 to 9.0. The enzyme had a K(m) of 190 micromolar for its substrate, 12-oxo-phytodienoic acid. The preferred reductant was NADPH, for which the enzyme exhibited a K(m) of 13 micromolar, compared with 4.2 millimolar for NADH. Reductase activity was low in the corn kernel but increased five-fold by the fifth day after germination and then gradually declined.

PubMed Disclaimer

References

    1. Can J Biochem Physiol. 1959 Aug;37(8):911-7 - PubMed
    1. Plant Physiol. 1981 Jan;67(1):92-7 - PubMed
    1. J Steroid Biochem. 1981 Jun;14(6):579-84 - PubMed
    1. Plant Physiol. 1970 Sep;46(3):445-53 - PubMed
    1. Biochem Biophys Res Commun. 1983 Mar 16;111(2):470-7 - PubMed

LinkOut - more resources