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. 1986 Feb;80(2):599-600.
doi: 10.1104/pp.80.2.599.

Measurement of the Enzyme-CO(2)-Mg Form of Spinach Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase

R E Seftor  1 , J T Bahr, R G Jensen
Affiliations

Measurement of the Enzyme-CO(2)-Mg Form of Spinach Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase

R E Seftor et al. Plant Physiol. 1986 Feb.

Abstract

When the amount of activation of ribulose 1,5-bisphosphate carboxylase has been measured, two forms of the enzyme, not one, are actually determined experimentally. Only the enzyme-activator CO(2)-Mg(2+) form can bind ribulose bisphosphate for reaction with substrate CO(2) or O(2). A method is presented which measures only this catalytically active form by stabilizing it with ribulose bisphosphate just before dilution and assay in Mg(2+)-free reaction medium.

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References

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