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. 1986 May;81(1):317-9.
doi: 10.1104/pp.81.1.317.

Isolation of Sperm Cells from the Pollen of Plumbago zeylanica

S D Russell  1
Affiliations

Isolation of Sperm Cells from the Pollen of Plumbago zeylanica

S D Russell. Plant Physiol. 1986 May.

Abstract

Intact sperm cells of Plumbago zeylanica were released from mature pollen grains near anthesis using osmotic shock with 20% sucrose. Sperm cell yields of up to 75% can be attained by differential centrifugation using a clinical centrifuge with concentrations of up to 8.8 x 10(6) cells/milliliter. Such ;gametoplasts' remain intact for up to 24 hours according to Evan's blue exclusion and can be used for characterization or physiological manipulation.

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References

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