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. 1986 May;81(1):8-11.
doi: 10.1104/pp.81.1.8.

Amino Acid transport in protoplasts isolated from soybean leaves

C D Vernooy  1 , W Lin
Affiliations

Amino Acid transport in protoplasts isolated from soybean leaves

C D Vernooy et al. Plant Physiol. 1986 May.

Abstract

We isolated large quantities of mesophyll protoplasts from source and sink leaves of soybean plants and examined them for amino acid uptake. Accumulation of amino acids in isolated protoplasts was linear for at least 40 minutes. Uptake kinetics revealed the presence of both saturable and linear components. Increasing external pH decreases the uptake. The uncoupler, carbonyl cyanide p-trifluoromethoxyphenylhydrazone at 15 micromolar inhibited and fusicoccin at 10 micromolar stimulated amino acid uptake. Our data are consistent with a proton-cotransport mechanism for the uptake of l-glutamine and alpha-amino isobutyric acid into soybean mesophyll cells.

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References

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