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. 1986 Oct;82(2):411-6.
doi: 10.1104/pp.82.2.411.

Identification of granule-bound starch synthase in potato tubers

G H Vos-Scheperkeuter  1 , W de Boer, R G Visser, W J Feenstra, B Witholt
Affiliations

Identification of granule-bound starch synthase in potato tubers

G H Vos-Scheperkeuter et al. Plant Physiol. 1986 Oct.

Abstract

Starch granules isolated from potato (Solanum tuberosum L.) tubers were extracted with sodium dodecyl sulfate and the extract was analyzed. A major protein with a molecular weight of 60,000 daltons was detected. This protein was purified by preparative sodium dodecyl sulfate-gel electrophoresis and specific antibodies were prepared. The anti-60-kilodalton antibodies obtained (a) cross-reacted with the waxy proteins of both maize (Zea mays L.) and grain amaranth (Amaranthus hypochondriacus L.), and (b) inhibited starch synthase activity in partially digested starch granules of the grain amaranth. This evidence strongly suggests that the major 60-kilodalton protein present in potato starch granules represents the granule-bound starch synthase.

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References

    1. Cell. 1983 Nov;35(1):225-33 - PubMed
    1. Anal Biochem. 1977 May 1;79(1-2):104-9 - PubMed
    1. J Mol Biol. 1984 Jun 5;175(4):511-28 - PubMed
    1. Genetics. 1981 Oct;99(2):275-84 - PubMed
    1. Arch Biochem Biophys. 1980 Oct 15;204(2):578-88 - PubMed

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