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. 1987 Feb;83(2):232-4.
doi: 10.1104/pp.83.2.232.

High speed HPLC analysis of polyamines in plant tissues

H J Walter  1 , J M Geuns
Affiliations

High speed HPLC analysis of polyamines in plant tissues

H J Walter et al. Plant Physiol. 1987 Feb.

Abstract

A high speed high performance liquid chromatography (HPLC) method for the quantification of putrescine, spermidine, and spermine in biological samples is described. The dansylation is followed by a sample cleanup. The isocratic HPLC-analysis with acetonitrile:H(2)O (72:28 volume/volume) on 10 centimeter long, 3 micrometer octadecyl silica columns (3 millimeter inner diameter) takes only 4.5 minutes. By our method about 100 analyses can be done in 1 day.

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References

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