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. 1991 Apr;5(4):214-7.
doi: 10.1002/rcm.1290050415.

Conformational changes in proteins probed by hydrogen-exchange electrospray-ionization mass spectrometry

V Katta  1 , B T Chait
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Conformational changes in proteins probed by hydrogen-exchange electrospray-ionization mass spectrometry

V Katta et al. Rapid Commun Mass Spectrom. 1991 Apr.

Abstract

Hydrogen-exchange electrospray-ionization mass spectrometry is demonstrated to be an effective new method for probing conformational changes of proteins in solutions. The method is based on the mass spectrometric measurement of the extent of hydrogen/deuterium exchange that occurs in different protein conformers over defined periods of time. Results are presented in which hydrogen-exchange electrospray-ionization mass spectrometry is used to probe conformational changes in bovine ubiquitin induced by the addition of methanol to aqueous acidic solutions of the protein.

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