Responses of Rj(1) and rj(1) Soybean Isolines to Inoculation with Bradyrhizobium japonicum

Abstract

We evaluated the symbiotic phenotypes of nodulation-restrictive and normal soybean isolines by inoculating Clark (genotypically Rj(1)Rj(1)) and mutant Clark-rj(1) (genotypically rj(1)rj(1)) seedlings in plastic growth pouches. Nodules first appeared on Clark seedlings inoculated with Bradyrhizobium japonicum USDA 94 after 6 days. The mean number of nodules per plant was 13.9 +/- 0.8 after 24 days. In contrast, Clark-rj(1) seedlings first nodulated at 12 days, and the mean number of nodules per plant was only 1.7 +/- 0.3 at 24 days. Segments from infectible zones of primary roots, i.e. near the position occupied by the root tip at the time of inoculation, were sectioned serially. Clark roots contained cortical cell divisions and a few infection threads in question mark-shaped root hairs by 2 days after inoculation. Typical nodules developed soon thereafter. Analogous serially sectioned segments from Clark-rj(1) roots lacked these responses. This prompted us to section nodules and adjacent tissues from other parts of Clark and Clark-rj(1) roots. Clark roots contained cortical cell divisions, many associated with infected root hairs. Cortical cell divisions occasionally were present in Clark-rj(1), and a few infection threads were visible in surface cells. The presence of infection threads within Clark-rj(1) nodules was confirmed by transmission electron microscopy. Thus, although B. japonicum USDA 94 fails to elicit the wild-type spectrum of responses in the infectible zones of primary roots, it can infect Clark-rj(1) via infection threads.