doi: 10.1104/pp.85.1.30.
Inhibition of na/h antiport activity in sugar beet tonoplast by analogs of amiloride
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- PMID: 16665675
- PMCID: PMC1054197
- DOI: 10.1104/pp.85.1.30
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Inhibition of na/h antiport activity in sugar beet tonoplast by analogs of amiloride
Plant Physiol.
1987 Sep.
Abstract
The effects of amiloride and a series of amiloride analogs have been tested on the Na(+)/H(+) antiport activity in intact vacuoles and tonoplast vesicles isolated from sugar beet cell suspension cultures. There is a competitive interaction between amiloride analogs and sodium. Substitution of one or both H-atoms of the 5-amino group of amiloride (apparent K(i) about 150 micromolar) resulted in a 3- to 200-fold increase in inhibitory potency of the antiport activity.
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