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. 1987 Dec;85(4):876-8.
doi: 10.1104/pp.85.4.876.

Procedure to Isolate Viable Sperm Cells from Corn (Zea mays L.) Pollen Grains

I Dupuis  1 , P Roeckel, E Matthys-Rochon, C Dumas
Affiliations

Procedure to Isolate Viable Sperm Cells from Corn (Zea mays L.) Pollen Grains

I Dupuis et al. Plant Physiol. 1987 Dec.

Abstract

Sperm cells were isolated from corn (Zea mays L.) tricellular pollen grains. They were released using a light osmotic chock, and separated from pollen contaminants (especially starch grains) by a Percoll gradient centrifugation. Isolated sperm cells (3 x 10(6) per milliliter) show a high viability score (90%) as demonstrated with the fluorochromatic reaction. They appeared as spherical cells which lack cell wall and plastids, and can be considered as haploid protoplasts.

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References

    1. Exp Cell Res. 1972 Jul;73(1):233-6 - PubMed
    1. Plant Physiol. 1986 May;81(1):317-9 - PubMed
    1. Stain Technol. 1970 May;45(3):115-20 - PubMed
    1. Plant Physiol. 1987 Mar;83(3):464-6 - PubMed
    1. Plasmid. 1980 Nov;4(3):233-55 - PubMed

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