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. 1989 Jun;90(2):512-5.
doi: 10.1104/pp.90.2.512.

Procedures for the Generation of Mature Chlamydomonas reinhardtii Zygotes for Molecular and Biochemical Analyses

D Wegener  1 , U Treier, C F Beck
Affiliations

Procedures for the Generation of Mature Chlamydomonas reinhardtii Zygotes for Molecular and Biochemical Analyses

D Wegener et al. Plant Physiol. 1989 Jun.

Abstract

Zygotes represent an important stage in the sexual cycle of the unicellular green alga Chlamydomonas reinhardtii. To study zygote germination at a molecular level, a protocol was elaborated for the generation of zygotes in large quantities and a method was developed for the extraction from zygotes of RNA that could be translated in vitro.

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