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. 1989 Dec;91(4):1507-11.
doi: 10.1104/pp.91.4.1507.

Phosphate Translocator of Mesophyll and Bundle Sheath Chloroplasts of a C(4) Plant, Panicum miliaceum L. : Identification and Kinetic Characterization

J Ohnishi  1 , U I Flügge, H W Heldt
Affiliations

Phosphate Translocator of Mesophyll and Bundle Sheath Chloroplasts of a C(4) Plant, Panicum miliaceum L. : Identification and Kinetic Characterization

J Ohnishi et al. Plant Physiol. 1989 Dec.

Abstract

The phosphate translocator was identified in the envelope membranes of both mesophyll and bundle sheath chloroplasts of Panicum miliaceum L. by labeling with [1,2-(3)H]1,2-(2,2' -disulfo-4,4' -diisothiocyano)diphenylethane ([(3)H]H(2)DIDS) and by using SDS-PAGE. Assay of (32)Pi uptake by the chloroplasts showed that the phosphate translocators of both types of chloroplasts have a higher affinity for phosphoenolpyruvate than the C(3) counterpart and can be regarded as C(4) types.

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