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. 1991 Oct;97(2):832-5.
doi: 10.1104/pp.97.2.832.

Hygromycin resistance gene cassettes for vector construction and selection of transformed rice protoplasts

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Hygromycin resistance gene cassettes for vector construction and selection of transformed rice protoplasts

Z Zheng et al. Plant Physiol. 1991 Oct.

Abstract

Hygromycin resistance gene cassettes were designed to facilitate vector construction for plant transformation. Unique EcoRI, Pstl, and Sacll sites in the coding sequence of a hygromycin B phosphotransferase gene (hph) from Escherichia coli were eliminated. The mutated hph genes were used to form gene cassettes flanked by EcoRI-Sacll-Kpnl-Hindlll sites. Hygromycin resistance of wild-type and mutated hph genes was indistinguishable in E. coli and rice protoplast growth assay.

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