Autotrophy in maize husk leaves : evaluation using natural abundance of stable isotopes

Abstract

The natural abundance of carbon and hydrogen isotopic composition, expressed as a delta(13)C value of plant dry matter and cellulose in the hypsophylls (husk leaves) of maize (Zea mays L.) was measured and compared with that of leaves and cobs. The delta(13)C values of outer hypsophylls were usually 2 to 3% per thousand more negative than leaves or other tissues, and became more negative with increasing chlorophyll content, indicating significant local C(3) pathway fixation of CO(2) in the outer hypsophylls. The deltaD values indicated a significant part of hypsophyll cellulose was derived from heterotrophic sources (sucrose from C(4) photosynthesis in other tissues). Isotopic mass balance calculations allowed quantitative estimation of these carbon sources and, in the samples examined, about 16% of hypsophyll cellulose was derived from local C(3) photosynthesis, about 62% from local C(4) photosynthesis, and about 22% from sucrose imported from other leaves.