Effect of diclofop on the membrane potentials of herbicide-resistant and -susceptible annual ryegrass root tips

Abstract

Electrophysiological measurements were made on root tip cells in the elongation zone of diclofop-methyl-resistant (SR4/84) and -susceptible (SRS2) biotypes of annual ryegrass (Lolium rigidum Gaud.) from Australia. The phytotoxic action of diclofop-methyl (methyl 2-[4-(2',4'-dichlorophenoxy)phenoxy]propanoate) on susceptible whole plants was completely reversed by a simultaneous application of 2,4-dichlorophenoxyacetic acid (dimethylamine salt). The phytotoxic acid metabolite, diclofop (50 micromolar), depolarized membrane potentials of both biotypes to a steady-state level within 10 to 15 minutes. Repolarization of the membrane potential occurred only in the resistant biotype following removal of diclofop. The resistant biotype has an intrinsic ability to reestablish the electrogenic membrane potential, whereas the susceptible biotype required an exogeneous source of IAA to induce partial repolarization. Both biotypes were susceptible to depolarization by carbonylcyanide-m-chlorophenylhy-drazone (CCCP), and their membrane potentials recovered upon removal of CCCP. A 15-minute pretreatment with p-chloromercuribenzenesulphonic acid (PCMBS) blocked the depolarizing action of diclofop in both biotypes. However, PCMBS had no effect on the activity of CCCP. The action of diclofop appears to involve a site-specific interaction at the plasmalemma in both Lolium biotypes to cause the increased influx of protons into sensitive cells. The differential response of membrane depolarization and repolarization to diclofop treatment may be a significant initial reaction in the eventual phytotoxic action of the herbicide.