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. 2006 May 10;128(18):6058-9.
doi: 10.1021/ja061261x.

Enantioselective alpha-hydroxylation of 2-arylacetic acid derivatives and buspirone catalyzed by engineered cytochrome P450 BM-3

Marco Landwehr  1 , Lisa Hochrein, Christopher R Otey, Alex Kasrayan, Jan-E Bäckvall, Frances H Arnold
Affiliations

Enantioselective alpha-hydroxylation of 2-arylacetic acid derivatives and buspirone catalyzed by engineered cytochrome P450 BM-3

Marco Landwehr et al. J Am Chem Soc. .

Abstract

Here we report that an engineered microbial cytochrome P450 BM-3 (CYP102A subfamily) efficiently catalyzes the alpha-hydroxylation of phenylacetic acid esters. This P450 BM-3 variant also produces the authentic human metabolite of buspirone, R-6-hydroxybuspirone, with 99.5% ee.

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