Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2006 May;44(5):1828-30.
doi: 10.1128/JCM.44.5.1828-1830.2006.

Rapid detection of Brucella spp. in blood cultures by fluorescence in situ hybridization

Affiliations

Rapid detection of Brucella spp. in blood cultures by fluorescence in situ hybridization

Nele Wellinghausen et al. J Clin Microbiol. 2006 May.

Abstract

Brucellosis is a severe systemic disease in humans. We describe a new 16S rRNA-based fluorescence in situ hybridization assay that facilitates rapid and specific detection of all human pathogenic species of Brucella and that can be applied directly to positive blood cultures.

PubMed Disclaimer

References

    1. Al Dahouk, S., H. Tomaso, K. Nockler, and H. Neubauer. 2004. The detection of Brucella spp. using PCR-ELISA and real-time PCR assays. Clin. Lab. 50:387-394. - PubMed
    1. Amann, R. I., B. J. Binder, R. J. Olson, S. W. Chisholm, R. Devereux, and D. A. Stahl. 1990. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations. Appl. Environ. Microbiol. 56:1919-1925. - PMC - PubMed
    1. Amann, R. I., L. Krumholz, and D. A. Stahl. 1990. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J. Bacteriol. 172:762-770. - PMC - PubMed
    1. Batchelor, B. I., R. J. Brindle, G. F. Gilks, and J. B. Selkon. 1992. Biochemical mis-identification of Brucella melitensis and subsequent laboratory-acquired infections. J. Hosp. Infect. 22:159-162. - PubMed
    1. Bricker, B. J., and S. M. Halling. 1994. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J. Clin. Microbiol. 32:2660-2666. - PMC - PubMed

Publication types

LinkOut - more resources