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. 2006 May;44(5):1884-6.
doi: 10.1128/JCM.44.5.1884-1886.2006.

PCR amplification of the IS6110 insertion element of Mycobacterium tuberculosis in fecal samples from patients with intestinal tuberculosis

Ramadass Balamurugan  1 , Subramanian Venkataraman, K R John, Balakrishnan S Ramakrishna
Affiliations

PCR amplification of the IS6110 insertion element of Mycobacterium tuberculosis in fecal samples from patients with intestinal tuberculosis

Ramadass Balamurugan et al. J Clin Microbiol. 2006 May.

Abstract

PCR amplification of insertion element IS6110 of Mycobacterium tuberculosis in fecal samples was evaluated in the diagnosis of intestinal tuberculosis (ITB). The numbers of samples that tested positive by PCR with SalI digestion were 16/18 untreated-ITB samples, 0/8 treated-ITB samples, 12/14 smear-positive pulmonary tuberculosis samples, and 0/30 control samples. The sensitivity, specificity, positive predictive value, and negative predictive value of fecal PCR were 88.8%, 100%, 100%, and 93.7%, respectively.

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Figures

FIG. 1.
FIG. 1.
Electrophoresis of PCR products on a 2% agarose gel (top panels and bottom right panel) or PCR product digest on a 3% agarose gel (bottom left panel). In each panel, lane M is the molecular weight marker. (Top left) Lanes 1 to 11 are DNA isolated from feces of control subjects. Lane 12 shows amplification of a 123-bp fragment from the positive-control DNA. (Bottom left) Digested PCR products, showing 66- and 57-bp fragments. Lanes 1 to 6, results from intestinal TB patients; lanes 7 and 8, results from pulmonary TB patients. Lane 9 is the positive control. (Top right) Lanes 1 to 11 show results from various intestinal TB patients, while lane 12 is a positive control. (Bottom right) Lanes 1 to 11 show results from patients with smear-positive pulmonary TB, while lane 12 shows the positive control.
See this image and copyright information in PMC

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