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. 2006 May;72(5):3780-4.
doi: 10.1128/AEM.72.5.3780-3784.2006.

Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli

Guillaume Bruant  1 , Christine Maynard, Sadjia Bekal, Isabelle Gaucher, Luke Masson, Roland Brousseau, Josée Harel
Affiliations

Development and validation of an oligonucleotide microarray for detection of multiple virulence and antimicrobial resistance genes in Escherichia coli

Guillaume Bruant et al. Appl Environ Microbiol. 2006 May.

Abstract

An oligonucleotide microarray detecting 189 Escherichia coli virulence genes or markers and 30 antimicrobial resistance genes was designed and validated using DNA from known reference strains. This microarray was confirmed to be a powerful diagnostic tool for monitoring emerging E. coli pathotypes and antimicrobial resistance, as well as for environmental, epidemiological, and phylogenetic studies including the evaluation of genome plasticity.

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Figures

FIG. 1.
FIG. 1.
Signal-to-noise fluorescence ratios obtained for the E. coli reference strain EDL933. The signal-to-noise ratios (log2) presented in this graph are the means of the ratios obtained by three independent replicate hybridizations performed with DNA from strain EDL933. All oligonucleotides which had a signal-to-noise fluorescence ratio of greater than 2.0 (log2 > 1) were considered positive. Oligonucleotides with a signal-to-noise ratio less than 2.0 (log2 < 1) were considered negative. For the negative results, only those for oligonucleotides specific to the green fluorescent protein (GFP) gene, Arabidopsis spp., Shigella flexneri, and C. freundii are shown.
See this image and copyright information in PMC

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