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. 2006 May 5;22(3):415-22.
doi: 10.1016/j.molcel.2006.03.014.

Asf1 mediates histone eviction and deposition during elongation by RNA polymerase II

Marc A Schwabish  1 , Kevin Struhl
Affiliations
Free article

Asf1 mediates histone eviction and deposition during elongation by RNA polymerase II

Marc A Schwabish et al. Mol Cell. .
Free article

Abstract

Histones are rapidly evicted and deposited during transcription by RNA polymerase (Pol) II, but a factor that mediates histone eviction in vivo has not yet been identified. Here, we show that the histone chaperone Asf1 associates with promoters and coding regions of transcriptionally active genes. Asf1 mediates histone H3, but not H2B, eviction and deposition during Pol II elongation, suggesting that nucleosome assembly and disassembly occur in a stepwise fashion. Lastly, Asf1 inhibits internal initiation from cryptic promoters within coding regions. These results strongly suggest that Asf1 functions as an elongation factor to disassemble and reassemble histones during Pol II elongation.

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