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Comparative Study
. 2006 Jul 15;451(2):141-8.
doi: 10.1016/j.abb.2006.03.030. Epub 2006 Apr 19.

Kinetic analysis of the effects of monovalent cations and divalent metals on the activity of Mycobacterium tuberculosis alpha-isopropylmalate synthase

Affiliations
Comparative Study

Kinetic analysis of the effects of monovalent cations and divalent metals on the activity of Mycobacterium tuberculosis alpha-isopropylmalate synthase

Luiz Pedro S de Carvalho et al. Arch Biochem Biophys. .

Abstract

Mycobacterium tuberculosis alpha-isopropylmalate synthase (MtIPMS) is a member of the family of enzymes that catalyze a Claisen-type condensation. In this work we characterized the monovalent and divalent specificity of MtIPMS using steady-state kinetics. The monovalent cation dependence of the kinetic parameters of substrates and divalent metals indicates that K+ is the likely physiological activator. K+ acts most likely as an allosteric activator, and exerts part of its effect through the catalytic divalent metal. The divalent metal specificity of MtIPMS is broad, and Mg2+ and Mn2+ are the metals that cause the highest activation. Interestingly, Zn2+, first assigned as the catalytic metal, inhibits the enzyme with submicromolar affinity. The features of monovalent cation and divalent metal activation, as well as the inhibition by Zn2+ and Cd2+, are discussed in light of the kinetic and structural information available for MtIPMS and other relevant enzymes.

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