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. 2005 Jul 29;38(1-2):107-114.
doi: 10.1016/j.vibspec.2005.02.015.

Infrared spectroscopic characterization of mineralized tissues

Adele L Boskey, Richard Mendelsohn

Infrared spectroscopic characterization of mineralized tissues

Adele L Boskey et al. Vib Spectrosc. .

Abstract

Vibrational spectroscopy (Infrared and Raman), and in particular micro-spectroscopy and micro-spectroscopic imaging has been used to characterize developmental changes in bone and other mineralized tissues, to monitor these changes in cell cultures, and to detect disease and drug-induced modifications. Examples of the use of infrared micro-spectroscopy and micro-spectroscopic imaging are discussed in this review.

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Figures

Fig. 1
Fig. 1
Hyperspectral images of bone mineral properties: in normal human cortical bone (a) typical spectrum from a single image pixel, (b) image of the mineral distribution in the biopsy, (c) image of the matrix distribution in the biopsy, (d) image of carbonate distribution, (e) image of mineral:matrix ratio, (f) image of crystallinity and (g) image of collagen cross link ratio. Note: all images are corrected for the presence of the embedding media, PMMA.
Fig. 2
Fig. 2
Images of mineralizing chondrocyte (left) and osteoblast (right) cultures: (a) mineral:matrix ratio, (b) crystallinity and (c) collagen cross-link ratio.
Fig. 3
Fig. 3
Image of a pathologic deposit removed from a patient with juvenile dermatomyositis: (a) mineral distribution, (b) lipid distribution, (c) mineral:matrix ratio (d) collagen cross-link ratio and (e) crystallinity distribution.
Fig. 4
Fig. 4
Pixel historgrams for the average distribution of mineral:matrix, crystallinity and collagen cross link ratio in normal and osteoporotic biopsies.
See this image and copyright information in PMC

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