Oligo(A) not coded by DNA generating 3'-terminal heterogeneity in a lambda phage RNA
- PMID: 167007
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Oligo(A) not coded by DNA generating 3'-terminal heterogeneity in a lambda phage RNA
J Biol Chem.
.
Free article
Abstract
An RNA species from Escherichia coli infected with phage lambda was purified by hybridization to lambda 1-strand DNA and shown to have variable length. This variability was due to a variable number of adenylate residues attached to the 3' end of the molecule. Pancreatic RNase treatment of the RNA-DNA hybrid removed the 3'-terminal adenylate residues, generating a homogeneous RNA molecule terminating with -Up. The results indicate the presence of adenylate residues not coded by the DNA template at the terminus of this transcript from intact cells.
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