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. 2006 May;12(5):719-24.
doi: 10.3201/eid1205.050446.

Universal genotyping in tuberculosis control program, New York City, 2001-2003

Carla M Clark  1 , Cynthia R Driver, Sonal S Munsiff, Jeffrey R Driscoll, Barry N Kreiswirth, Benyang Zhao, Adeleh Ebrahimzadeh, Max Salfinger, Amy S Piatek, Jalaa Abdelwahab
Affiliations

Universal genotyping in tuberculosis control program, New York City, 2001-2003

Carla M Clark et al. Emerg Infect Dis. 2006 May.

Abstract

In 2001, New York City implemented genotyping to its tuberculosis (TB) control activities by using IS6110 restriction fragment length polymorphism (RFLP) and spoligotyping to type isolates from culture-positive TB patients. Results are used to identify previously unknown links among genotypically clustered patients, unidentified sites of transmission, and potential false-positive cultures. From 2001 to 2003, spoligotype and IS6110-based RFLP results were obtained for 90.7% of eligible and 93.7% of submitted isolates. Fifty-nine (2.4%) of 2,437 patient isolates had false-positive culture results, and 205 genotype clusters were identified, with 2-81 cases per cluster. Cluster investigations yielded 57 additional links and 17 additional sites of transmission. Four additional TB cases were identified as a result of case finding initiated through cluster investigations. Length of unnecessary treatment decreased among patients with false-positive cultures.

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Figures

Figure
Figure
Median days for submission and turnaround time by laboratory, New York City, 2001–2003. PHL, public health laboratory; RFLP, restriction fragment length polymorphism.
Figure A1
Figure A1
Summary of tuberculosis (TB) genotyping activities, New York City, 2001–2003. NYC, New York City; LJ, Lowenstein-Jensen; RFLP, restriction fragment length polymorphism.
See this image and copyright information in PMC

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