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. 2006 Jun;12(6):1025-8.
doi: 10.3201/eid1206.060015.

Mixed Cryptosporidium infections and HIV

Vitaliano Cama  1 , Robert H Gilman, Aldo Vivar, Eduardo Ticona, Ynes Ortega, Caryn Bern, Lihua Xiao
Affiliations

Mixed Cryptosporidium infections and HIV

Vitaliano Cama et al. Emerg Infect Dis. 2006 Jun.

Abstract

Mixed Cryptosporidium infections were detected in 7 of 21 patients with a diagnosis of rare Cryptosporidium canis or C. felis infections; 6 patients were infected with 2 Cryptosporidium spp. and 1 patient with 3 species. Mixed infections may occur more frequently than previously believed and should be considered when assessing cryptosporidiosis.

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Figures

Figure
Figure
Multilocus polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) analysis of specimens previously identified as Cryptosporidium canis and C. felis. A) Agarose gel electrophoresis of PCR-amplified products of specimens previously identified as C. canis (lanes 1–3) and C. felis (lanes 4 and 5) with molecular tools based on the small subunit (SSU) rRNA, dihydrofolate reductase (DHFR), and Cryptosporidium oocyst wall protein (COWP). Molecular markers in all photos are 100-bp ladders. B) RFLP analysis of DHFR-based PCR amplification products using BpuA I restriction enzyme; lanes 1, 4, and 5 are C. hominis; lane 2 is C. parvum; and lane 3 is C. meleagridis. C) RFLP analysis of COWP-based PCR amplification products using Rsa I restriction enzyme; lanes 1, 4, and 5 are C. hominis; lane 2 is C. parvum; and lane 3 is C. meleagridis. D) RFLP analysis of the SSU-based PCR products using restriction enzymes Ssp I (left) and Vsp I (right); the combined patterns for lanes 1 to 3 correspond to C. canis and lanes 4 and 5 to C. felis.
See this image and copyright information in PMC

References

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